About Us > Faculty & Staff > Faculty > Susan Sharfstein
Susan Sharfstein
Associate Professor of Nanobioscience
Listen to Professor Sharfstein discuss using nanoparticles for gene delivery in 3D cell assays on the August 2010 ACS Nano podcast
Contact Information:
Education:
- Ph.D. in Chemical Engineering, University of California, Berkeley, 1993
- B.S. with honors in Chemical Engineering, California Institute of Technology, Pasadena, CA, 1987
Honors and Awards:
- Marquis Who's Who in America, 2010
- RPI Class of '51 Outstanding Teaching Award, 2007
- RPI School of Engineering Excellence in Education Award, 2007
- CAREER: National Science Foundation Early Career Development Award, 2000
- Inducted into Sigma Xi at University of Toledo, 1997
- National Institutes of Health Postdoctoral Fellowship, 1993
Description of research:
The focus of the research in the Sharfstein laboratory is on understanding the role of culture conditions and cell physiology on use of living systems for industrially relevant processes. Our primary area of interest is the use of mammalian cell systems for the production of therapeutic proteins and carbohydrates. We use the tools of modern cell and molecular biology along with “omics” to probe physiological states, with an objective of optimizing production systems both from an engineering perspective (e.g. culture conditions) as well as from a biological perspective (cellular and metabolic engineering). Current projects are focused on the production of heparin (a critically important anticoagulant drug) in Chinese hamster ovary (CHO) cells, characterizing CHO cell clones producing recombinant monoclonal antibodies to identify factors that affect productivity, the effects of osmolarity on monoclonal antibody production in CHO cells, and the effects of growth rate on cell physiology in Escherichia coli.
We are also interested in developing new tools using nanotechnology to better understand cultured cells and bioprocesses. Current projects are focused on patterning cells on surfaces for high throughput delivery of silencing RNA and development of novel biosensors for use in bioreactors.
Publications:
T.R. Kiehl, D. Shen. S.F. Khattak, Z. Lee, and S.T. Sharfstein, Observations of cell-size dynamics under osmotic stress, Cytometry Part A, accepted
A.C. Baughman, S.T. Sharfstein, and L.L. Martin, A Flexible State-space Approach for the Modeling of Metabolic Networks I: Development of Mathematical Methods, Metabolic Engineering, 13: 125-137 (2011). doi:10.1016/j.ymben.2010.12.002
A.C. Baughman, S.T. Sharfstein, and L.L. Martin, A Flexible State-space Approach for the Modeling of Metabolic Networks II: Advanced Interrogation of Hybridoma Metabolism, Metabolic Engineering, 13: 138-149 (2011). doi:10.1016/j.ymben.2010.12.003
H. Zhang, M-Y. Lee, M.G. Hogg, J.S. Dordick, and S.T. Sharfstein, Gene delivery in three-dimensional cell cultures by superparamagnetic nanoparticles, ACS Nano, 4: 4733–4743 (2010). http://pubs.acs.org/doi/abs/10.1021/nn9018812
D. Shen, T. R. Kiehl, S.F. Khattak, Z. Li, A. He, I.M. Neuhaus, P.S. Kayne, V. Patel, and S. T. Sharfstein, Transcriptomic Responses to Osmotic Stress: A Study of Industrial Fed-Batch CHO Cell Cultures, Biotechnology Progress, 26: 1104-1115 (2010). http://www3.interscience.wiley.com/journal/123276644/abstract
A.C. Baughman, X. Huang, S.T. Sharfstein, and L.L. Martin, On the Dynamic Modeling of Mammalian Cell Metabolism and mAb Production, Computers in Chemical Engineering, 34: 210-222 (2010). doi:10.1016/j.compchemeng.2009.06.019
J.H. Nam, M. Ermonval, and S.T. Sharfstein, The effects of microcarrier culture on recombinant CHO cells under biphasic hypothermic culture conditions, Cytotechnology, 59: 81-91 (2009). DOI: 10.1007/s10616-009-9196-x
S. Barua, S, A. Joshi, A. Banerjee, D. Matthews, S. T. Sharfstein, S. Cramer, R. Kane, K. Rege, Parallel Synthesis and Screening of Polymers for Non-Viral Gene Delivery, Molecular Pharmaceutics, 6: 86-97 (2009). http://pubs.acs.org/doi/abs/10.1021/mp800151j
Z. Jiang and S.T. Sharfstein, Characterization of gene localization and accessibility in DHFR-amplified CHO cells, Biotechnology Progress, 25: 296-300 (2009). http://www3.interscience.wiley.com/journal/121634088/abstract
J.H. Nam, F.Zhang, R.J. Linhardt, and S.T. Sharfstein, Effects of culture conditions on the glycosylation of SEAP produced by rCHO cells in a controlled bioreactor: Biotechnology and Bioengineering, 100: 1178-1192 (2008). http://www3.interscience.wiley.com/journal/117928542/abstract
S.T. Sharfstein Advances in Cell Culture Process Development: Tools and Techniques for Improving Cell Line Development and Process Optimization: Biotechnology Progress, 24: 727-734 (2008). http://www3.interscience.wiley.com/journal/121398262/abstract
Z. Jiang and S.T. Sharfstein, Sodium Butyrate Stimulates mAb Over-expression in CHO Cells by Improving Gene Accessibility, Biotechnology and Bioengineering, 100: 189-194 (2008). http://www3.interscience.wiley.com/journal/117346235/abstract
A. Venkiteshwaran, P. Heider, S. Matosevic, A. Bogsnes, A. Staby, S. Sharfstein, and G. Belfort Optimized removal of soluble host cell proteins for the recovery of met-human growth hormone inclusion bodies from Escherichia Coli cell lysate using crossflow microfiltration, Biotechnology Progress, 23: 667-672. (2007). http://www3.interscience.wiley.com/journal/121398909/abstract
J.H. Nam M. Ermonval, and S.T. Sharfstein, Cell attachment to microcarriers affects growth, metabolic activity, and culture productivity in bioreactor culture, Biotechnology Progress, 23: 652 -660 (2007). http://www3.interscience.wiley.com/journal/121398951/abstract
D. Shen and S.T. Sharfstein, Genome-Wide Analysis of the Transcriptional Response of Murine Hybridomas to Osmotic Shock, Biotechnology and Bioengineering, 93: 132-145 (2006). http://www3.interscience.wiley.com/journal/112098359/abstract
Z. Jiang, Y. Huang, and S.T. Sharfstein, Regulation of Recombinant Monoclonal Antibody Production in Chinese Hamster Ovary Cells: A Comparative Study of Gene Copy Number, mRNA Level and Protein Expression, Biotechnology Progress, 22: 313-318 (2006). One of the 10 most accessed articles of 2006 and 20 most cited papers in 2006-2007 from Biotechnology Progress. http://www3.interscience.wiley.com/journal/121410373/abstract
K.M. McNeeley, Z. Sun, and S.T. Sharfstein, Techniques for Dual Staining of DNA and Intracellular Immunoglobulins in Murine Hybridoma Cells: Applications to Cell-Cycle Analysis of Hyperosmotic Cultures, Cytotechnology, 48: 15-26 (2005). http://www.springerlink.com/content/74u2447k61ux850n/fulltext.pdf
Z. Sun, R. Zhou, S. Liang, K.M. McNeeley, and S.T. Sharfstein, Hyperosmotic Stress in Murine Hybridoma Cells: Effects on Antibody Transcription, Translation, Posttranslational Processing, and the Cell Cycle, Biotechnology Progress, 20: 576-589 (2004). http://www3.interscience.wiley.com/journal/121400780/abstract
J. Faghihi, X. Jiang, R. Vierling, S. Goldman, S. Sharfstein, J. Sarver, and P. Erhardt, Reproducibility of the High-Performance Liquid Chromatographic Fingerprints Obtained from Two Soybean Cultivars and a Selected Progeny, Journal of Chromatography A, 915: 61-74 (2001).
D.C. MacLaren, S.S. Gambhir, N. Satyamurthy, J.R. Bario, S. Sharfstein, T. Toyokuni, L.Wu, A.J. Berk, S.R. Cherry, M.E. Phelps, H.R. Herschman, Repetitive, Non-invasive Imaging of the Dopamine D2 Receptor as a Reporter Gene in Living Animals. Gene Therapy 6:785-791 (1999).
Gambhir, S.S., Barrio, J.R., Wu, L., Iyer, M., Namavari, M., Satyamurthy, N., Parrish, C., MacLaren, D.C., Borghei, A.R., Bauer, E., Green, L.A., Sharfstein, S., Berk, A.J., Cherry, S.R., Phelps, M.E., and Herschman, H.R. Imaging of adenoviral directed herpes simplex virus Type 1 thymidine kinase reporter gene expression in mice with ganciclovir. J. Nucl. Med. 39:2003-2011 (1998). (Awarded 3rd place for Outstanding Basic Science Investigations for 1998)
L.A. Green, S.S. Gambhir, A. Srinivasan, P.K. Banerjee, C.K. Hoh, S.R. Cherry, S. Sharfstein, J. Bario, H. Herschman, and M.E. Phelps, Non-Invasive Methods for Quantitating Blood Time-Activity Curves from Mouse PET Images Obtained with Fluorine-18-Flurodeoxyglucose, J. Nuclear Medicine, 39:729-734 (1998).
A. Mancuso, S.T. Sharfstein, E.J. Fernandez, D.S. Clark, and H.W. Blanch, Effect of Extracellular Glutamine Concentration Primary and Secondary Metabolism of a Murine Hybridoma: An In Vivo 13C Nuclear Magnetic Resonance Study, Biotechnology and Bioengineering, 57:172-186 (1998).
S.T. Sharfstein, S.J. Van Dien, and J.D. Keasling, Modulation of the Phosphate-Starvation Response in Escherichia coli by Genetic Manipulation of the Polyphosphate Pathways, Biotechnology and Bioengineering, 51: 434-437 (1996).
S.T. Sharfstein and J.D. Keasling, Polyphosphate Metabolism in Escherichia coli, Annals New York Academy of Science, 745:77-91 (1994).
A. Mancuso, S.T. Sharfstein, S.N. Tucker, D.S. Clark, and H.W. Blanch, Examination of Primary Metabolic Pathways in a Murine Hybridoma with Carbon-13 Nuclear Magnetic Resonance Spectroscopy, Biotechnology and Bioengineering, 44:563-585 (1994).
S.T. Sharfstein, S.N. Tucker, A. Mancuso, H.W. Blanch, and D.S. Clark, Quantitative In Vivo NMR Studies of Hybridoma Metabolism, Biotechnology and Bioengineering, 43:1059-1074 (1994).
S.T. Sharfstein, B. Gaillard, H.W. Blanch, and D.S. Clark, Functional Differentiation and Primary Metabolism of Mouse Mammary Epithelial Cells in Extended Batch and Hollow Fiber Culture, Biotechnology and Bioengineering, 40:672-680 (1992).
G. Agopian, P. James, S. Knee, and S. Sharfstein, Novel Technique for Determining Magnet Cleanliness - NMR Spectroscopy, IEEE Transactions on Magnetics, 23:3602 - 3604 (1987).
Invited Book Chapters:
Mikael R. Andersen, Jong Hyun Nam, and Susan T. Sharfstein, “Protein Glycosylation: Analysis, Characterization, and Engineering” The Encyclopedia of Industrial Biotechnology, Bioprocess, Bioseparation, and Cell Technology, Michael Flickinger, ed. John Wiley & Sons, Inc., in press (2010)
Susan T. Sharfstein and Christian Kaisermayer, "Microcarrier culture" in The Encyclopedia of Industrial Biotechnology, Bioprocess, Bioseparation, and Cell Technology, Michael Flickinger, ed. John Wiley & Sons, Inc., p 3450-3468 (2010).
Susan T. Sharfstein, Duan Shen, Thomas R. Kiehl and Rui Zhou, "Molecular Response to Osmotic Shock" in Cellular Engineering v.5: Systems Biology, M. Al-Rubeai and M. Fussenegger, ed. Springer NL p213-236 (2007).
Susan T. Sharfstein and Jong Hyun Nam, "Protein Glycosylation: Analysis and Characterization" in Bioseparation and Bioprocessing, 2nd ed., G. Subramanian, ed. Wiley-VCH p631-662 (2007).
Peer-reviewed Conference Proceedings:
S. Sharfstein, B. Barquera, M. Hanna, Biotechnology and Bioprocessing and Microbiology Laboratory Courses: A Model for Shared Used of Instructional Laboratories between Engineering and Science, Proceedings of 2008 American Society for Engineering Education Annual Conference and Exposition (2008). http://soa.asee.org/paper/conference/paper-view.cfm?id=7430
A.B. Samuels, S. Sharfstein, and L.L. Martin, Optimization of mAb Synthesis via the Application of an IDEAS Formulation, Proceedings of the 2004 AIAA/ISSMO Multidisciplinary Analysis and Optimization Conference (2004).
S. Sharfstein and P. Relue, Biotechnology and Bioprocessing Laboratory for Chemical Engineering and Bioengineering, Proceedings of the 2001 American Society for Engineering Education Annual Conference & Exposition (2001). http://soa.asee.org/paper/conference/paper-view.cfm?id=15569